cheese
ingredient / ingredient-cheese
チーズ
Cheese is a ripened dairy product in which chymosin and proteinases and peptidases from starter cultures drive both the generation and breakdown of bitter peptides through casein degradation.
Photographs (captions in Japanese)

A photograph of a wedge cut from a type of cheese (a Swiss-type aged cheese with eyes). The eyes in the cut face and the side can be seen against a white background. It is not a photograph representing a specific region or brand.
Shown: No applicable taxon (cross-section of a Swiss-type aged cheese)Part / state: A wedge cut from a Swiss-type cheese (with eyes, i.e., holes), with the cut face and side visible
Photo: Renee Comet (Photographer) / Public domain / original image / ledger's original image
Scope and provenance of the photo
Identification: Identified as labelled in the source. The source is a general "cheese" photograph provided by the U.S. National Cancer Institute (NCI), with no variety or type name or origin. It is not treated as the standard form for the broad name "cheese" as a whole.
Image shown: The 1920 px version from Wikimedia Commons. No cropping or color changes by NIGAMI LAB. For display, a WebP capped at 1200 px wide is served; the ledger's original image can be checked from the link above.
On this page (7 sections)
Reading the bitterness
The bitterness of aged cheese is described as arising in part from peptides (fragments of several linked amino acids) formed when casein, a milk protein, breaks down. A study of the water-soluble fraction of aged Gouda identified 16 bitter peptides derived from casein, of which 11 were from β-casein and 6 from αs1-casein. Another study of aged sharp Cheddar identified the 5 peptides with the greatest bitterness intensity; all were derived from β-casein.
However, the concentration of any individual peptide alone does not strongly account for bitterness intensity. In reduced-fat Cheddar, correlations between concentrations of individual peptides and trained-panel bitterness scores were limited to r=0.56–0.58, whereas an index combining two peptides reached r=0.84. The authors also acknowledge that other peptides may contribute to bitterness. Cheese bitterness cannot be attributed to a single peptide.
Consistent with this view, a reconstructed model made by adding 5 peptides at the same concentrations to young mild Cheddar was reported not to differ significantly in bitterness intensity from authentic aged sharp Cheddar. One peptide, GPVRGPFPIIV, increased 28.7-fold during aging and is described as a major contributor to bitterness. Another study reported a correlation of 0.803 at 180 days of aging between the concentration of a related β-casein-derived peptide and trained-panel bitterness ratings. By 270 days, however, the correlation had fallen to 0.554; the authors raised the possibility that other bitter peptides or bitterness-mitigating compounds were involved.
Cheese bitterness also depends on the type of enzyme carried by the lactic starter. In an experiment with reduced-fat Cheddar, cheeses were made with Lactococcus lactis strains differing in the specificity of lactocepin, an extracellular proteinase, and a trained panel of 14 rated bitterness. Cheese made with a strain lacking the proteinase scored 1.8, whereas cheeses made with strains carrying lactocepin scored 3.5–4.7; the difference was statistically significant at 6 months of aging. This was a model experiment using reduced-fat cheese and single-strain starters, however, and may not apply directly to ordinary cheeses.
In an exploratory experiment on bitterness-masking activity targeting cheese, among 71 commercial brands the natural white-mold-type Baraka cheese showed the strongest activity. In a sensory test in which Baraka was eaten immediately before taking a sip of beer, the weakening of bitterness was greater than with any of Gouda, Brie, or Ricotta, and when the free-fatty-acid concentrations of the 4 cheeses were compared, the total concentration was also higher in the order of stronger activity. When Baraka's oil was fractionated, the fraction whose main component was free fatty acids masked quinine's bitterness most strongly, and the same suppressive effect was confirmed with a mixture that mimicked the cheese's fatty-acid composition.
Home-cooking practices that combine cheese with bitter leafy greens are also recorded. A recipe introduction for a salad combining watercress with roasted almonds and Parmigiano mentions the combination of watercress's bitterness and the almonds' toasty aroma, and states that Parmigiano's saltiness and umami are used to finish the dish without adding salt. A recipe introduction for a dish in which shungiku (garland chrysanthemum) is dressed with grated cheese and ground sesame states that the cheese mellows the bitterness of the shungiku. Both are the cooks' own introductions and are not experiments that measured the interaction of bitterness and umami.
Sources for this overview
Sensomics mapping and identification of the key bitter metabolites in Gouda cheese
Checked for this overview: Checked the identification of 16 bitter peptides in aged Gouda and the breakdown by casein origin.
Identification of bitter peptides in aged cheddar cheese
Checked for this overview: Checked that the 5 most intensely bitter peptides identified in aged Cheddar were derived from β-casein. / Checked the reconstruction experiment finding no significant bitterness difference between the 5-peptide model and the authentic cheese.
Checked for this overview: Checked the difference in correlations for single-peptide and combined-peptide indicators. / Checked the differences in bitterness scores among lactocepin specificity groups.
Checked for this overview: Checked how correlations between a single peptide's concentration and bitterness changed with aging time.
Identification of bitterness-masking compounds from cheese
Checked for this overview: Used for the screening of 71 brands, the sensory comparison of 4 cheeses against beer, and the correspondence with free-fatty-acid concentration / Used for the figures on quinine bitterness suppression by the fatty-acid fraction and the mixed fatty-acid solution
Checked for this overview: Used for the quotation from the recipe introduction for watercress, almonds, and Parmigiano
Checked for this overview: Used for the quotation from the introduction to the shungiku dressed with sesame and cheese
Follow the connections
cheese →— bitterness derives in par…→ bitter peptides
bitterness →reduces taste preference for→ cheese
cheese →has a defining sensory theme→ bitterness →can be measured separately …→ astringency
Hypotheses and recipe candidates for this entry are on the Japanese page.
Structured claims — open a section to read each claim's confidence, evidence and limits
Sensory (8)
Scope: Aged Gouda and aged sharp Cheddar (water-soluble extracts).
Note: The explanatory power of any single peptide alone is limited: in Broadbent 2002, the correlation coefficients between a single peptide's concentration and the bitterness score stayed at 0.56 and 0.58, versus r=0.84 for a combined 2-peptide index (the sum of each concentration divided by its highest value in the data set) (see limits).
Show data
ingredient-cheese —derives_bitterness_in_part_from→ compound-class-bitter-peptidesScope: Aged sharp Cheddar versus young mild Cheddar. A taste-reconstitution experiment.
Note: The sequences are as given in the abstract (residue numbers are not written because they are not given in the abstract).
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compound-class-bitter-peptides —reconstitute_bitterness_of→ ingredient-cheeseScope: Experimental fresh cheese (CoF) made by fermenting high-protein concentrated milk reconstituted from micellar casein powder (8.5% protein, about 74 mM calcium). Bitterness rated by 12-13 members per session of a 25-member trained panel.
Note: This paper's conclusion section summarizes that "the PrtP-negative strains did not produce bitterness," but this is at odds, in the strength of the assessment, with the figures in the results section (see the corresponding evidence's limits line), and the discussion states that cheese made with LTH 7112 was significantly more bitter than the directly acidified control.
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ingredient-cheese —develops_bitterness_depending_on→ (the claim sentence above)Scope: Cheddar cheese, aged 6 months. Sensory evaluation was carried out at the 6-month point.
Note: 2026-10-21 (full-text re-evaluation, batch 2): in the full text, the abstract (bitterness enhanced) and the sensory results in the text (weaker than without nisin) run in opposite directions; as neither can be settled, the claim was lowered from supported to preliminary.
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ingredient-cheese —develops_bitterness_depending_on→ (the claim sentence above)Scope: Trained-panel sensory evaluation of 25 commercially available U.S. shredded Cheddar brands (2 lots each), followed by consumer preference ratings of 10 representative brands by 151 consumers; analyzed with analysis of variance, principal component analysis, and external preference mapping.
Note: Bitterness was identified as one driver of overall dislike. A powdery appearance also lowered appearance and overall liking ratings.
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sensation-bitter —reduces_taste_preference_for→ ingredient-cheeseScope: A study relating sensory profiles before and after heating to physicochemical parameters in 4 categories of AOP/PDO blue cheeses from France's Massif Central: Fourme d'Ambert, Fourme de Montbrison, Bleu d'Auvergne, and Bleu des Causses. Along with saltiness and sourness, bitterness was recorded as one of the basic tastes distinguishing these 4 categories after heating, and correlations with physicochemical parameters were also shown.
Note: The paper does not describe bitterness as either a 『欠陥』 (in translation: "defect") or a 『望ましい風味』 (in translation: "desirable flavor"). It treats it neutrally as an attribute distinguishing categories; this entry likewise does not overstate its significance. No peer-reviewed paper addressing bitterness in Gorgonzola itself was found, so the study of French AOP cheeses is included as a related blue-cheese lineage (see the corresponding research lead).
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ingredient-cheese —has_defining_sensory_theme→ sensation-bitterScope: Layer: food. A human sensory panel (n=7) using 4 commercial cheeses and beer. Baraka was selected through a preliminary screening of 71 cheese brands.
Note: The correspondence between the total free-fatty-acid concentration and masking activity is a descriptive trend across 4 samples, and the fatty-acid composition ratio itself was nearly the same among the 4 cheeses (all shared the feature that oleic acid, palmitic acid, myristic acid, and stearic acid accounted for 60% or more of total free fatty acids). Details of the 71-brand screening results themselves are in the Supporting Information, and the main text gives no figures.
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ingredient-cheese —can_reduce_perceived_bitterness_of→ sensation-bitterScope: Layer: solution (fractions of the cheese's oily extract; a mixture mimicking the cheese's fatty-acid composition). Human sensory evaluation (a method comparing against a 7-point scale of standard quinine solutions).
Note: The evaluation of the mixed fatty-acid solution had large variance among panelists (ANOVA F-test p=0.001). The authors cite the low solubility of the saturated fatty acids (myristic acid, palmitic acid, and stearic acid) as one cause. With oleic acid alone (0.5 mM), bitterness was suppressed down to the equivalent of 0.0032-0.0038%, and the variance among panelists was also small (F-test p=0.131) (a different part of the same experiment as the claim in the existing concept-taste-mixture-suppression entry).
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ingredient-cheese —can_reduce_perceived_bitterness_of→ sensation-bitterHow processing and cooking change it (9)
Scope: 50% reduced-fat Cheddar, a single isogenic starter, a trained panel (n=14).
Note: A result from a model system (reduced-fat, single-strain starter). Extrapolation to ordinary cheese is limited.
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ingredient-cheese —develops_bitterness_depending_on→ (the claim sentence above)Scope: A proposal envisaging high-protein fermented dairy products, including concentrated fresh cheese.
Note: Based on the statement 『a sensible selection and combination of prtP-positive and -negative starter strains might be a useful tool...and should be investigated in future studies』. This is not a demonstrated method.
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ingredient-cheese —can_be_debittered_by→ (the claim sentence above)Scope: Reduced-fat Cheddar cheese, ripened for 150 days. Chemical, microbiological, and sensory analyses were performed at regular intervals.
Note: The abstract does not give the specific score difference for "markedly lower," and the full text was not checked, so it is not written here.
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ingredient-cheese —can_be_debittered_by→ (the claim sentence above)Scope: Aged Cheddar cheese compared across 4 groups: control, D. hansenii alone, Y. lipolytica alone, and both species combined (the country of the experiment is not given in the abstract).
Note: The specific sensory scale and panel size are not stated in the abstract.
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ingredient-cheese —develops_bitterness_depending_on→ (the claim sentence above)Scope: As above: the group combining both species in a 4-group comparative experiment.
Note: The abstract does not explicitly say 『苦味を避けた』 (in translation: "avoided bitterness"). It only describes this group as having 『good strong flavour』, in contrast to the single-species groups; the absence of a mention of bitterness cannot establish 『苦味が生じなかった』 (in translation: "that no bitterness developed"). (2026-10-22, expansion stage N5 stage C batch 6: Codex's check against the source confirmed that the abstract only describes good flavor for the combined group and does not say bitterness was absent, so debittering rests on a reading of this abstract alone; lowered from supported to preliminary.)
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ingredient-cheese —can_be_debittered_by→ (the claim sentence above)Scope: Same as above (Cheddar cheese, aged 6 months).
Note: The combination that produced 『脱苦味効果』 (in translation: "a debittering effect") (Lb. casei + nisin-producing strain) and the combination rated as having 『風味強度が最高』 (in translation: "the highest flavor intensity") (Lb. casei + liposome-encapsulated nisin) are distinguished in the abstract, and this note is written so as not to conflate them. NFB, which ranked first for overall quality in the text, had Lb. casei added alone without nisin, and ISNB, combined with the nisin-producing strain, ranked below NF. (2026-10-21, expansion stage N5 stage C batch 5: Codex's check against the source confirmed that the sensory test was an overall-quality rating by 5 people with no numerical bitterness intensity and no test, and that milk heat treatment also differed with and without Lb. casei; lowered from supported to preliminary.)
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ingredient-cheese —can_be_debittered_by→ (the claim sentence above)Scope: The same 1996 review of cheese bitterness.
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ingredient-cheese —can_be_debittered_by→ (the claim sentence above)Scope: Layer: recipe (an introduction by a cook). Not a sensory experiment. Posted on the Japan Umami Seasoning Association's recipe site (Seiko Ogawa, 2020-04-02).
Note: What the original text explicitly links is the combination of 「クレソンの苦味」 (in translation: "the bitterness of the watercress") and 「アーモンドの香ばしさ」 (in translation: "the toasty aroma of the almonds"), and there is no direct statement that the cheese weakened the bitterness of the watercress. The role of the cheese is explained in the introduction as a substitute for salt and umami so the dish can be finished without added salt. This is recorded as the cook's practical knowledge, not as sensory data demonstrating the interaction of bitterness and umami.
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ingredient-cheese —is_used_in→ sensation-bitterScope: Layer: recipe (an introduction by an instructor). Not a sensory experiment.
Note: This is a dressed dish involving salt-boiling, rinsing in water, ground sesame, soy sauce, and sugar, and is not a comparison isolating the cheese alone. The assessment 「まろやかにしてくれます」 (in translation: "mellows it") is stated by the instructor as a composite impression that includes the cheese's saltiness, umami, and fat content, and is not an experiment that measured the interaction of bitterness and umami.
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ingredient-cheese —can_reduce_perceived_bitterness_of→ sensation-bitter