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Topic hub — 2026-10-21

Do cooking and additions reduce the bitterness and egumi of spinach?

A caveat first: the studies of whether cooking reduces it measured egumi (an acrid harshness left on the tongue and throat), not bitterness. This page does not treat egumi and bitterness results as the same and does not present reduced egumi as reduced bitterness. Spinach is often said to become easier to eat when boiled in plenty of water and soaked. What do studies that changed cooking, growing or additions and had people compare spinach bitterness or egumi say? This page lines up the included studies together with their conditions: cultivar and growing, water amount and salt, and who rated them on what scale. It then separates what was established within one experiment, what differences between studies might explain, and what this comparison alone cannot separate. It is about perceived taste, not eating or health advice.

On this page (10 sections)
  1. What this page establishes
  2. What the included studies allow us to say
  3. Bitterness ratings at a glance
  4. Conditions and results, study by study
  5. What was established within an experiment
  6. Candidate explanations for the differences
  7. What is not known — what this comparison alone cannot separate
  8. How studies and rows were counted
  9. What we looked at
  10. Read in the encyclopedia
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What this page establishes

What the included studies allow us to say

Bitterness ratings at a glance

Human bitterness ratings. Values for "Group compared" and "Comparison" are as reported in the tables or text (values shown only in figures are marked as such). Both egumi rows and bitterness rows are included (the detailed table for each row shows which was measured). For studies read only as abstracts, only what the abstract states is shown; where values are only in figures, only whether differences were significant is shown. "Direction" shows which sample was more bitter; whether the difference is significant is shown by the test in the same "Direction / test" column. Select a study name to jump to its detailed table.

Results in which people rated bitterness only. Where each value comes from (a table or a reading from a figure) is shown in its row; scales and methods differ between studies, so compare within a row, not across rows.

StudyComparison and conditionMeasured asSample comparedComparisonDirection / test
Izumi 2005a20 vs 5 times the water
Spring and autumn, 1% salt in the water in every condition
5-point scale (+2 very strong to −2 none, 12–14 people)no significant difference detected (values only in a figure)values only in a figureDirection not given
Tested, not significant
Izumi 2005b3% salt vs distilled water
Concentration test (cv. Sunlight): 1, 2 or 3% salt vs distilled water
5-point scale (positive = weaker egumi, 10–21 people)tended weaker (values only in a figure)values only in a figureDirection not given
Tested, not significant
Izumi 2005b3% refined or table salt vs distilled water
Salt-type test (cv. Tonic), 3% salt in the water
5-point scale (positive = weaker egumi, 10–21 people)egumi significantly weaker (values only in a figure)values only in a figureDirection not given
Tested, only some pairs significant
Horie 2006Ethanol vs water fraction
Fractions of a leaf extract separated on a solid phase (at a 20-fold dilution of the leaf)
symbol ratings (small panel including the authors)+ (bitter)− (not bitter)Sample compared more bitter
No test reported
Izumi 2004W town vs Y district
Origins compared within the same month (same cooking, 1% salt in the water)
5-point scale (+2 very strong to −2 none, 9 people)stronger (values only in a figure)values only in a figureSample compared higher
Tested, only some pairs significant
Izumi 2008Growing method, cultivar and growth stage
Autumn, 12 conditions after boiling (4 plots × 3 stages)
mean on a 5-point scale (+2 very strong to −2 none, 15–16 people)range −0.88 to 0.27not applicable (12 conditions compared)Direction not given
Tested, not significant
Izumi 2005c100 mM NaCl vs none
5 mM oxalic acid solution (not spinach)
5-point scale (8 people)significantly lower (values only in a figure)values only in a figureComparison higher
Tested, significant
Batziakas 2019Open field vs commercial
Raw leaves (not cooked)
mean on a 0–15 intensity scale (6 trained assessors)3.02.4Sample compared more bitter
Tested, significant
Bakke 2018With vs without sugar or salt
Broccoli, spinach and kale purées (3 vegetables reported together)
not given (not in the abstract)lower (abstract wording)values not in the abstractComparison more bitter
No test reported

Conditions and results, study by study

For each study, the conditions for the samples, preparation, serving and who rated them, and the results. Each row is one study × one measured result, so different comparisons in the same study are separate rows. Under each row we give where it is in the paper and whether it can be used to judge whether cooking, growing or additions change the bitterness or egumi people perceive in spinach, with the reason. Nothing is left blank: "not given", "not read" and "not applicable" are kept apart.

Izumi 2005a sensory only

ゆで水量の違いがホウレンソウの食味やシュウ酸ならびにカリウム含量に及ぼす影響. 日本調理科学会誌 38(4):343-349 — doi:10.11402/cookeryscience1995.38.4_343

How deeply checked
Checked in full text — Full text via J-STAGE (Figs 1–7, Table 3); egumi values only in figures, viewed but not transcribed Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Spinach from the same field in Sendai (spring cv. Altair, autumn cv. Mahoroba)
Processing and cooking
Boiled 1 min in 5, 7, 10 or 20 times the leaf weight of water (1% salt in every condition), then soaked 5 min in water
How it was served or eaten
Boiled leaves
Who rated, on what scale
12–14 female students and staff, 5-point scale (+2 very strong egumi to −2 none), rating egumi plus colour, flavour, texture and overall; two-way ANOVA
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Egumi (acrid harshness, sensory)20 vs 5 times the water
Spring and autumn, 1% salt in the water in every condition
no significant difference detected (values only in a figure)values only in a figure5-point scale (+2 very strong to −2 none, 12–14 people)Direction not given
Tested, not significant
No significant difference among water amounts for any of the 5 attributes in spring or autumn (which does not mean the same). Free oxalate in the same leaves differed between 5 and 20 times by about 100 mg% in spring and 30 in autumn; the drop in water temperature also differed (about 6 °C at 5 and 7 times, 4 °C at 20 times) Where: Figs 1 and 2 (egumi), Fig. 5 (water temperature), Figs 6 and 7 (oxalate)
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Egumi compared across water amounts; bitterness was not measured and every condition had 1% salt

Izumi 2005b sensory only

食塩添加ゆでホウレンソウのえぐ味の感じ方とシュウ酸および無機成分含量との関連. 日本家政学会誌 56(1):15-21 — doi:10.11428/jhej1987.56.15

How deeply checked
Checked in full text — Full text via J-STAGE (Figs 1–4, 7, 8; Tables 1, 2); egumi values only in figures Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Concentration test with cv. Sunlight, salt-type test with cv. Tonic (different cultivars and origins)
Processing and cooking
Boiled 1 min in 10 times the leaf weight of water, soaked 5 min; concentration test with 0, 1, 2 or 3% salt in the water, salt-type test with 3% of Amashio (a branded sea salt), reagent NaCl, table salt or refined salt
How it was served or eaten
Boiled leaves
Who rated, on what scale
10–21 female students and staff, 5-point scale (weaker egumi scored positive); two-way ANOVA
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Egumi (acrid harshness, sensory)3% salt vs distilled water
Concentration test (cv. Sunlight): 1, 2 or 3% salt vs distilled water
tended weaker (values only in a figure)values only in a figure5-point scale (positive = weaker egumi, 10–21 people)Direction not given
Tested, not significant
No significant difference for any of the 5 attributes; egumi and colour tended to improve with more salt. The abstract reads as if salt reduced egumi, stronger than the concentration test in the text Where: Fig. 1
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Egumi compared across salt concentrations; bitterness was not measured
Egumi (acrid harshness, sensory)3% refined or table salt vs distilled water
Salt-type test (cv. Tonic), 3% salt in the water
egumi significantly weaker (values only in a figure)values only in a figure5-point scale (positive = weaker egumi, 10–21 people)Direction not given
Tested, only some pairs significant
Among the differences stated in the text, 3% refined salt and table salt gave weaker egumi than distilled water (p<0.01); the figure also marks differences between distilled water and reagent NaCl (p<0.05) and between Amashio (a branded sea salt) and table salt (p<0.05) and refined salt (p<0.01). No difference was detected between Amashio and distilled water. Total oxalate did not differ with salt (Figs 3 and 4), and the author suggests the weaker egumi reflects the salt itself rather than less oxalate Where: Figs 2–4, 7, 8
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Egumi compared across salt types; bitterness was not measured, and the cultivar and origin differ from the concentration test

Horie 2006 sensory only

ホウレンソウのえぐ味はシュウ酸に由来するか. 日本調理科学会誌 39(6):357-361 — doi:10.11402/cookeryscience1995.39.6_357

How deeply checked
Checked in full text — Full text via J-STAGE (Tables 1 and 3) Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Leaf blades of greenhouse spinach at a national vegetable research institute (cv. Okame, Parade)
Processing and cooking
Leaves blended with water and the extract separated on a solid phase into water-eluted and ethanol-eluted fractions (fractionation in cv. Parade only; the Table 3 liquids were each at a 20-fold dilution of the leaf blade)
How it was served or eaten
Liquids tasted
Who rated, on what scale
A very small panel including the authors (size not given); bitterness and oxalate taste recorded separately with symbols (− or a number of +); no statistical test reported
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Bitterness intensity (sensory)Ethanol vs water fraction
Fractions of a leaf extract separated on a solid phase (at a 20-fold dilution of the leaf)
+ (bitter)− (not bitter)symbol ratings (small panel including the authors)Sample compared more bitter
No test reported
Oxalate was 4 mg/L in the ethanol fraction and 368 in the water fraction, with oxalate taste − and ++; bitterness and oxalate taste separated into different fractions. Panel size and tests are not given, and boiled leaves were not compared Where: Table 3
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Shows bitterness and oxalate taste come from different components, but no cooking or additions were compared; small panel, no test

Izumi 2004 sensory only

ホウレンソウ中のシュウ酸およびカリウム含量の季節変動と調理による変化. 日本調理科学会誌 37(3):268-272 — doi:10.11402/cookeryscience1995.37.3_268

How deeply checked
Checked in full text — Full text via J-STAGE (Table 1, Fig. 5); egumi values only in a figure Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Spinach from W town (greenhouse) and Y district of Sendai (mostly open field) in 9 months from May 2000 to March 2001 (no August or December, and no July for Y district)
Processing and cooking
Boiled 1 min in 10 times the leaf weight of water with 1% salt, soaked 1 min in 5 times the water and 4 min in fresh water, squeezed by hand to 80% of the original weight; same for all samples
How it was served or eaten
Boiled leaves
Who rated, on what scale
9 female college students and staff, 5-point scale (+2 very strong to −2 none)
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Egumi (acrid harshness, sensory)W town vs Y district
Origins compared within the same month (same cooking, 1% salt in the water)
stronger (values only in a figure)values only in a figure5-point scale (+2 very strong to −2 none, 9 people)Sample compared higher
Tested, only some pairs significant
W town was stronger in almost every month, significant at the 1% level in May and November and at 5% in September and March; within-month differences in free oxalate between origins were 260 mg% in November and 267 in March. The 2 origins also differed in greenhouse vs open field and in cultivar Where: Fig. 5
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Egumi compared between origins within the same month; the origins also differ in greenhouse vs open field and cultivar, and bitterness was not measured

Izumi 2008 sensory only

秋期ホウレンソウの品種, 栽培条件, 生育期間の違いによる有機酸や糖含量等の変動および茹で調理による変化, 食味との関連. 日本調理科学会誌 41(2):126-133 — doi:10.11402/cookeryscience1995.41.2_126

How deeply checked
Checked in full text — Full text via J-STAGE (Tables 1 and 3) Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Autumn-sown cv. Upright (open field, rain shelter, glasshouse) and cv. Asagiri (open field) at 3 growth stages
Processing and cooking
Boiled 1 min in 7 times the leaf weight of water with 1% salt, soaked 1 min in 5 times the water and 4 min in fresh water, squeezed by hand to 80% of the original weight; same for all samples
How it was served or eaten
Boiled leaves
Who rated, on what scale
15–16 female students, 5-point scale (+2 very strong to −2 none); two-way ANOVA
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Egumi (acrid harshness, sensory)Growing method, cultivar and growth stage
Autumn, 12 conditions after boiling (4 plots × 3 stages)
range −0.88 to 0.27not applicable (12 conditions compared)mean on a 5-point scale (+2 very strong to −2 none, 15–16 people)Direction not given
Tested, not significant
No significant difference in egumi under any condition (which does not mean the same); ratings were skewed towards weak. Oxalate after boiling fell with growth (300–440 mg% early, 140–230 late) Where: Table 3
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Egumi compared by growing method, cultivar and stage; bitterness not measured

Izumi 2005c sensory only

シュウ酸のえぐ味に及ぼす塩化ナトリウムならびに粘性の影響. 日本調理科学会誌 38(4):362-367 — doi:10.11402/cookeryscience1995.38.4_362

How deeply checked
Checked in full text — Full text via J-STAGE (Figs 1–7); values only in figures Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Not spinach: reagent oxalic acid and potassium oxalate solutions (such as 5 mM and 100 mM)
Processing and cooking
NaCl (50 or 100 mM) or citrus pectin at 0.2% added
How it was served or eaten
Solutions at 20 °C tasted with the whole mouth
Who rated, on what scale
8–13 female students and staff, 5-point scale (+2 very strong to −2 none)
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Egumi (acrid harshness, sensory)100 mM NaCl vs none
5 mM oxalic acid solution (not spinach)
significantly lower (values only in a figure)values only in a figure5-point scale (8 people)Comparison higher
Tested, significant
p<0.01; NaCl 50 mM in 100 mM potassium oxalate also lowered egumi (13 people), and 0.2% citrus pectin lowered it in 5 mM oxalic acid (p<0.05) but not significantly in 50 mM potassium oxalate. NaCl at 50–100 mM is about 0.3–0.6%, a different amount from the 1–3% in boiling water Where: Figs 3–5
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Not usable — An experiment with oxalate solutions, not spinach leaves

Batziakas 2019 sensory only

Reducing food losses and improving the quality of locally produced spinach. Kansas State University doctoral dissertation, Chapter 4, pp.127-157 — source

How deeply checked
Checked in the full text of the underlying thesis — Full text of chapter 4 of the doctoral thesis (Table 4.2) Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Organic cv. Corvair grown in Kansas (open field, high tunnel) and commercial organic baby spinach (cultivar and harvest date unknown)
Processing and cooking
Not cooked. Grown samples were washed, drained, stored at 0.5 °C and harvested 5 days before evaluation; the commercial sample was bought 2 days before and stored at 0.5 °C and 95% RH (washing not stated). Post-harvest handling not matched
How it was served or eaten
2–3 raw unseasoned leaves (about 10 g) at room temperature
Who rated, on what scale
6 assessors with over 120 hours of descriptive training, 0–15 intensity scale, 3 replicates; ANOVA and Fisher's LSD
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Bitterness intensity (sensory)Open field vs commercial
Raw leaves (not cooked)
3.02.4mean on a 0–15 intensity scale (6 trained assessors)Sample compared more bitter
Tested, significant
Overall p=0.010; the high tunnel (2.8) was not found to differ significantly from either. The commercial sample came from a different origin, with unknown cultivar and time from harvest to evaluation. There was no egumi attribute, and related attributes such as astringency did not differ significantly Where: Table 4.2
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — Raw-leaf bitterness compared by growing; not a comparison of cooking or additions, and the commercial sample differs in many ways

Bakke 2018 sensory only

Mary Poppins was right: Adding small amounts of sugar or salt reduces the bitterness of vegetables. Appetite 126:90-101 — doi:10.1016/j.appet.2018.03.015

How deeply checked
Abstract only — Abstract only (full text paywalled) Checked by: LLM (one extracted, another checked against the source; no human check), 2026-10-21.
Samples
Broccoli, spinach and kale purées (preparation not in the abstract)
Processing and cooking
Experiment 1: 0, 0.6, 1.2 or 1.8% sugar, or 0 or 0.2% salt; Experiment 2: 0 or 2% sugar; Experiment 3: 0, 1 or 2% sugar
How it was served or eaten
Not given (not in the abstract)
Who rated, on what scale
Experiment 1: descriptive analysis by 9 adults with more than 20 h of green-vegetable-specific training; Experiment 2: forced choice (bitterness) by 84 adults; Experiment 3: liking rated by 99 adults on a 9-point scale; test names not in the abstract
MeasuredComparison and conditionSample comparedComparisonUnitDirection / test
Bitterness intensity (sensory)With vs without sugar or salt
Broccoli, spinach and kale purées (3 vegetables reported together)
lower (abstract wording)values not in the abstractnot given (not in the abstract)Comparison more bitter
No test reported
The abstract says small amounts of sugar or salt lowered bitterness in all 3 vegetables; values and tests for spinach alone are not in the abstract Where: Abstract
For whether cooking, growing or additions change the bitterness or egumi people perceive in spinach: Limited — The abstract reports adding sugar or salt and comparing bitterness, but values and tests for spinach alone were not checked in full text

What was established within an experiment

Only results where the same panel compared changed conditions within one study.

Two identical small pans, with a dish of salt beside the right one. Under each is a plate of boiled spinach and a purple mark, darker on the left and lighter on the right.

Boiled in distilled water

Boiled in 3% salt water

Purple mark: egumi (harsh, acrid taste), not bitterness

A study in which spinach (cultivar Tonic) was boiled for 1 minute in water 10 times its weight and then soaked in water for 5 minutes, comparing distilled water with water containing 3% refined or table salt. Egumi was significantly weaker in the leaves boiled in salt water. The mark shows the order of egumi only and is not a measure of bitterness. The right side stands for the direction shared by the refined-salt and table-salt conditions; it is not a mix of the two salts, nor does it mean the two had equal values. The tested Amashio salt (a branded sea salt) showed no significant difference from distilled water, and an experiment with another cultivar comparing salt concentrations found no difference either, so more salt cannot be said to work better. The figure also makes no claim that oxalic acid was reduced. The purple is not a real colour.

Source: Izumi 2005b (Izumi 2005b in the table of conditions and results by study, the salt-type experiment)

Illustration: illustration desk (PurpleHeron)

Candidate explanations for the differences

Conditions that differ between studies, or between samples, and could explain differences in egumi or bitterness. Several conditions differ at once, so which one mattered is unknown. These are candidates, not causes.

What is not known — what this comparison alone cannot separate

How studies and rows were counted

The number of evidence rows is not the number of studies. Table rows, ledger evidence rows, papers and research groups are counted separately.

What we looked at

The sources examined for this question, why each was included or excluded, and which full texts could not be reached. We searched J-STAGE and Europe PMC for studies in which people rated spinach bitterness or egumi while cooking, growing or additions were varied, and added the sources already in the lab's ledger (CiNii was not searched). Paywalled studies are marked as read from the abstract only.

SourceDecisionWhy
ゆで水量の違いがホウレンソウの食味やシュウ酸ならびにカリウム含量に及ぼす影響 (2005) doiIncluded in the tableWater amount and egumi; read in full
食塩添加ゆでホウレンソウのえぐ味の感じ方とシュウ酸および無機成分含量との関連 (2005) doiIncluded in the tableSalt in boiling water and egumi; read in full
ホウレンソウのえぐ味はシュウ酸に由来するか (2006) doiIncluded in the tableBitterness and oxalate taste separated; read in full
ホウレンソウ中のシュウ酸およびカリウム含量の季節変動と調理による変化 (2004) doiIncluded in the tableEgumi by origin and month; read in full
秋期ホウレンソウの品種, 栽培条件, 生育期間の違いによる有機酸や糖含量等の変動および茹で調理による変化, 食味との関連 (2008) doiIncluded in the tableEgumi by growing method, cultivar and stage; read in full
シュウ酸のえぐ味に及ぼす塩化ナトリウムならびに粘性の影響 (2005) doiIncluded in the tableSalt or pectin added to oxalate solutions; read in full
Reducing food losses and improving the quality of locally produced spinach (2019)Included in the tableRaw-leaf bitterness by growing; read in the full thesis chapter
Mary Poppins was right: Adding small amounts of sugar or salt reduces the bitterness of vegetables (2018) doiIncluded in the tableSugar or salt and purée bitterness; abstract only
Masking Vegetable Bitterness to Improve Palatability Depends on Vegetable Type and Taste Phenotype (2013) doiExcludedA study of asparagus, Brussels sprouts and kale, not including spinach

Read in the encyclopedia

Source ledger

Each study's source can be reached from its section above. All of the lab's sources are listed in the source ledger.

How to cite this page

Aoki, M. (NIGAMI LAB) (2026). Do cooking and additions reduce spinach bitterness?. https://nigamilab.com/en/topics/spinach-bitter-egumi/

Each claim card has a stable anchor (#clm-…). The dataset is CC BY 4.0; see the data page. Dataset DOI: 10.5281/zenodo.22739101